T cell receptor repertoire usage in cancer as a surrogate marker for immune responses. Bias in the αβ T-cell repertoire: implications for disease pathogenesis and vaccination. Advances and applications of immune receptor sequencing in systems immunology. The αβ T cell repertoire comes into focus. TCR repertoire as a novel indicator for immune monitoring and prognosis assessment of patients with cervical cancer. Using an in silico meta-repertoire generated from 108 replicates, we found that one genomic DNA-based method and two non-unique molecular identifier (UMI) RNA-based methods were more sensitive than UMI methods in detecting rare clonotypes, despite the better clonotype quantification accuracy of the latter.Ĭui, J.-H. Results from the 5′ RACE-PCR methods were consistent among themselves but differed from the RNA-based multiplex-PCR results. Low RNA input generated non-representative repertoires. Most methods showed a lower ability to capture TRA than TRB diversity. We found marked differences in accuracy and intra- and inter-method reproducibility for T cell receptor α (TRA) and T cell receptor β (TRB) TCR chains. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapid amplification of complementary DNA ends (RACE)-polymerase chain reaction (PCR) and three multiplex-PCR approaches, when applied to the same T cell sample. Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear.
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